5-Amino-1MQ 50mg

Description

5-Amino-1MQ 50mg — NNMT Inhibitor Research Peptide

5-Amino-1-methylquinolinium (5-Amino-1MQ) is a small molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme studied for its role in cellular energy metabolism and adipocyte differentiation. NNMT catalyzes the methylation of nicotinamide using S-adenosyl-L-methionine (SAM) as a methyl donor, and its overexpression has been observed in metabolically relevant tissues in preclinical research models.

Mechanism of Action

5-Amino-1MQ inhibits NNMT enzymatic activity, which has been studied for its downstream effects on:

• NAD+ Salvage Pathway: NNMT inhibition may increase intracellular nicotinamide availability, potentially supporting NAD+ biosynthesis through the salvage pathway.
• SAM-Dependent Methylation: By reducing NNMT consumption of SAM, inhibition may preserve SAM availability for other methyltransferase reactions involved in epigenetic regulation and polyamine synthesis.
• Adipocyte Metabolism: Published research has examined NNMT expression in adipose tissue and the effects of its inhibition on lipogenesis-related gene expression in cell culture models.

Published Research

Neelakantan et al. (2018) published in Biochemical Pharmacology describing the development of selective NNMT inhibitors including 5-Amino-1MQ. The study characterized the compound binding profile, selectivity, and effects in cellular assays. Subsequent studies have examined NNMT inhibition in the context of metabolic research using diet-induced models in rodents.

Specifications

• Content: 50mg per vial
• Molecular Weight: 159.19 g/mol
• Form: Lyophilized powder
• Purity: ≥98% (HPLC verified)
• Storage: -20°C lyophilized, protected from light and moisture
• Solubility: Soluble in water and DMSO

Research Applications

• NNMT enzymatic activity and inhibition studies
• NAD+ metabolism and salvage pathway research
• Adipocyte differentiation and lipogenesis pathway investigations
• SAM-dependent methylation and epigenetic regulation studies
• Cellular energy metabolism research

References

1. Neelakantan H, et al. Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse high fat diet-induced obesity in mice. Biochemical Pharmacology. 2018;147:141-152.
2. Kraus D, et al. Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature. 2014;508(7495):258-262.
3. Pissios P. Nicotinamide N-methyltransferase: more than a vitamin B3 clearance enzyme. Trends in Endocrinology & Metabolism. 2017;28(5):340-353.

This product is sold strictly for in-vitro research and laboratory use only. Not for human consumption. Researchers are responsible for compliance with all applicable regulations.